Figure 1.

Hematopoietic cell transplant (HCT) changes the community composition of the lung microbiota but does not alter community diversity. (A) Principal component (PC) analysis of untreated control mice and HCT mice demonstrates changes in lung community composition (P = 0.002, permutational multivariate ANOVA). (B) This change in community composition was driven by enrichment with the Firmicutes phylum (P = 0.0024) and relative loss of the Bacteroidetes phylum (P = 0.0003). (C) Yet, rarefaction analysis revealed that community richness—the number of unique species per specimen—was not changed by HCT (P > 0.05). (D) Similarly, the lungs of HCT-treated mice did not differ from those of untreated mice in the Shannon diversity index (P > 0.05). OTU = operational taxonomic unit.