Skip to main content
. 2018 Nov 27;9(93):36585–36602. doi: 10.18632/oncotarget.26379

Figure 6. Endothelial cell tube formation assay.

Figure 6

HMEC-1 cells seeded on a Geltrex™ support were stimulated with EL4 (A), K12 (B) or MDA-MB-231 (C) supernatants. Graphs show the quantification of the arithmetic means ± SD of EC tube formation of three independent experiments. Representative micrographs show the formation of the endothelial network after 6 h of seeding on a Geltrex™ support and stimuli with tumor cells supernatants. C−: DMEM; C+: ECs stimulated with 100 ng/ml of VEGF; BC: supernatant of untreated tumor cells. *p < 0.05 *** p < 0.001 vs. untreated cells.