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. Author manuscript; available in PMC: 2019 Jan 10.
Published in final edited form as: J Control Release. 2017 Nov 21;269:405–422. doi: 10.1016/j.jconrel.2017.11.031

Fig. 6.

Fig. 6.

Effects of PIN1 knock-down on inhibition of ATRA against HuH7 and PLC cell growth. Growth curve of (a) HuH7 and (d) PLC cells expressing empty vector or PIN1 shRNA was established by MTT assay. HuH7 cells expressing empty vector (b) or PIN1 shRNA (c), and PLC cells expressing empty vector (e) or PIN1 shRNA (f) were treated with 20 μM free ATRA or ATRA-PLLA particles for 24 h, 48 h, and 72 h, and then the cell viability was assayed using MTT method, (g) HuH7 cells expressing empty vector or PIN1 shRNA, and (h) PLC cells expressing empty vector or PIN1 shRNA were treated with blank PLLA particles (0.25 mg/mL), free ATRA and ATRA-PLLA particles (ATRA concentration 20 μM) for 72 h, and then the PIN1 expression was determined by Western blotting analysis. β-Actin was used as an internal control. Untreated cells were set as control. Values are the mean ± standard deviation of triplicate determinations; *p < 0.05, **p < 0.01, ***p < 0.001.