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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 Dec 24;142(4):1173–1184. doi: 10.1016/j.jaci.2017.10.043

FIG 1.

FIG 1.

GATA2 is critical for connective tissue mast cell differentiation and IgE/mast cell-mediated anaphylaxis. A, Flow analysis of peritoneal cavity mast cells (MC) and bone marrow basophils (Ba). Total number of cells (mean ± SEM, n=3 mice) (right panel). B, Toluidine blue staining of mast cells (magnification 3×40; insert, 3×100). Mast cells are indicated by arrows. Average number of mast cells in ten randomly selected fields (mean ± SEM, n=3 mice). C, PCA analysis, mean ± SEM, n = 3 mice; D, PSA analysis, mean ± SEM, n = 6 mice. E, ELISA analysis of histamine, mean ± SEM, n=3 samples from 3 individual mice. F, ELISA analysis of MMCP-1 (mean ± SEM, n=3 samples from 3 individual mice). G, PSA analysis of the Gata2f/fMcpt5-Cre mice reconstituted BMMCs (mean ± SEM, n=5 mice). Statistical differences were analyzed by student’s t test (A, B, C, E, F) or by ANOVA (D, G). *: p<0.05; **: p<0.01; ***: p<0.001.