The transfection factor MITF, but not AHR, AHRR or BHLHE40 regulates
the Hdc gene expression. qPCR analysis of the
Hdc mRNA expression and ELISA analysis of histamine
content in BMMCs (A), PCMCs (C), and human mast
cell line LAD2 (D) with knocked down Mitf mRNA
(ShMitf) or with shRNA control (ShCTRL) (mean ±
SEM, triplicates); B. qPCR analysis of the Hdc
mRNA expression in BMMCs with knocked down Ahr mRNA
(ShAhr), Ahrr mRNA
(ShAhrr), and Bhlhe40 mRNA
(ShBhlhe40) or with shRNA control (ShCTRL) (mean
± SEM, triplicates). The left, middle, and right panels each show
three independent experiments. FIG E4A related to FIG 3A; FIG E4B related to FIG 3B; FIG E4C related to FIG
3D; FIG E4D related to FIG
3E.