MITF drives the −8.8 enhancer activity. A,
Luciferase reporter gene analysis of Hdc enhancer
activities in CFTL-15 cells with knocked down Mitf mRNA or
shRNA control (mean ± SEM, triplicates). B. Luciferase
reporter gene analysis of the Hdc enhancer activities in
HEK293 cells co-transfected with vector control or the MITF expression
construct (mean ± SEM, triplicates). C. Luciferase
reporter gene analysis of the activity of Hdc enhancers
that have a mutated MITF binding site(s) (mean ± SEM, triplicates).
The left and right panels (A, B, and C) or the
upper and lower panel (D) each show two independent
experiments. FIG E12A related to FIG
6A; FIG E12B related to FIG 6B;
FIG E12C related to FIG 6C.