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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 Dec 24;142(4):1173–1184. doi: 10.1016/j.jaci.2017.10.043

FIG 4.

FIG 4.

Identification of putative Hdc enhancers. A, BMMCs were used for ChIP-seq (duplicate samples were used for H3K4me1 ChIP-seq and triplicate samples were used for H3K27ac ChIP-seq). H3K4me1 and H3K27ac ChIP-seq data were analyzed with the Integrative Genomics Viewer. Red bars indicate putative Hdc enhancers that show significant H3K4me1 and H3K27ac modifications. RPM: reads per million nucleotides; E: enhancer, +0.3 indicates the distance from the beginning of the downstream Hdc enhancer to the TSS of the Hdc gene, and −8.8 indicates the distance from the beginning of the upstream Hdc enhancer to the TSS of the Hdc gene. B, Scheme of the luciferase constructions (upper panel) and luciferase reporter analysis of the Hdc enhancer activities in the CFTL-15 mast cell line_(lower panel). Error bars represent mean ± SEM, n=3 transfectants. HdcMP: Hdc minimal promoter.