MITF is sufficient to fully restore cKIT and FcεRI expression
but can only partially restore Hdc gene expression.
A, Flow analysis of cKIT and FcεRI expression in
Gata2−/− BMMCs overexpressing
MITF, GATA2 or control vector (CTRL). YFP+ GFP+ cell
populations are shown. Data represent three independent experiments with similar
results. B, qPCR analysis of the Hdc mRNA
expression in Gata2−/− BMMCs
overexpressing MITF, GATA2 or a control vector (CTRL). YFP+
GFP+ BMMCs were FACS-sorted for qPCR analysis. Error bars
represent mean ± SEM, n=3 samples from 3 independent experiments.