Table E1.
Primer sequences:
Primers for qPCR and
ChIP-qPCR | ||
---|---|---|
Gene | 5′ primer | 3′ primer |
Mitf | AACCGACAGAAGAAGCTGGA | TGATGATCCGATTCACCAGA |
Ahr | AGCAGCTGTGTCAGATGGTG | CTGAGCAGTCCCCTGTAAGC |
Ahrr | AGAGGAGGACCAGAAGCACA | TCTGTGCTCCAGTTCAGGTG |
Bhlhe40 | CATGAGAACACTCGGGACCT | TCTCTTTCAAGTCCACGGCT |
Hdc | TTCCAGCCTCCTCTGTCTGT | GGTATCCAGGCTGCACATTT |
Hprt | CTCATGGACTGATTATGGACAGGAC | GCAGGTCAGCAAACTTATAGCC |
Human HDC | TTCTGCCTGTGGATGACAAC | TAAGCAGCATCGATGTGGAG |
Human HPRT | CTCATGGACTAATTATGGACAGGAC | GCAGGTCAGCAAAGAATTTATAGCC |
HdcE+0.3BS1 ChIP | GGGGCCACAGTGTATGAAAC | AGGAAAATGTGTCCCACTGC |
HdcE+0.3BS2 ChIP | GAAGGGTTTCTGGTTGCTCA | CTTGTCACACGGCTTCCAC |
HdcE-8.8BS3&4 ChIP | GCAAAGCCTCAAGGTGGTAA | GAAGCTGCCCGAATACTTGT |
HdcE-8.8BS5 ChIP | CTGCAAGCTACATTTTCAGCA | TGTGAGAACAAACTGCACTGG |
Primers for
constructions | ||
Constructs | 5′ primer | 3′ primer |
pGL3-HdcMP | AATAAGATCTGGCTAAAGGAGGGGAATAGG | AATAAAGCTTGCATCTCTTATTTAAGGAGTTCCC |
pGL3-HdcMPE+0.3 | AATAGCTAGCGTAGATCAGAGTTTTAACAGGTCCCT | AATAAGA TCTCTTCTTCTTCTTCTTCTTTTTTTTTTTT |
pGL3-HdcMPE-8.8 | AATAGGTACCAACCAATTAGACTCCTCATGCCTT | AATAACGCGTAAGGAACTGATAAACGAGGGGTG |
BS3 Mut | GTTCTGCTGGAGACAATTAAATACAG | ATTACATCCTGTATTTAATTGTCTCCAG |
BS4 Mut | CAGCTGCAGGCGAATTAATGT | GTGAAAGCAACATTAATTCGCCT |
BS5 Mut | AAGCCGTTGGAGAATTAATTGCT | ACACAAACCCAAGCAATTAATTCTC |