Table 1.

The Methylated and Unmethylated Primer Sequences of RASSF1A and RB used in the Present Study

Gene name	Primer status	Primer sequence (5’ to 3’)	Ref.
RASSF1A	Unmethylated Forward	GGTTGTATTTGGTTGGAGTG	Matthaios et al., 2016
	Unmethylated Reverse	CTACAAACCTTTACACACAACA
	Methylated Forward	GTTGGTATTCGTTGGGCGC
	Methylated Reverse	GCACCACGTATACGTAACG
RB	Unmethylated Forward	GGGAGTTTTGTGGATGTGAT
	Unmethylated Reverse	ACATCAAAACACACCCCA	Liu et al., 2012
	Methylated Forward	GGGAGTTTCGCGGACGTGAC
	Methylated Reverse	ACGTCGAAACACGCCCCG