. 2018 Dec 12;13(12):e0207161. doi: 10.1371/journal.pone.0207161

Table 2. Primers used in this study.

Primers for knockout construction	S. aureus Mu50 Open Reading Frame	Sequence
MUT0224X	SAV0226	`GAAGTTGCTTCTGTTGGTCCTGCAATATCTTGC`
MUT0224AR	SAV0224	`GATGAATCGTACGATGCTCAATC`
MUT0223CF2		`ATCGTACGATTCATCGTCGACGGAATTATGATCTGCCCCGAGAC`
MUT0223Y	SAV0222	`ATTCGAAACAACTTTAAGGCG`
MUTO224AF		`AGTCAGGGATCCTTGAACCGTTC`
MUT0223CR	SAV0222	`AGTCAGGGTACCGGTGTTCCAATCAGTTGGTGG`
SAV0224-ermB-f		`AGTCAGGTCGACGAAGGAGGGATTCGTCATGTTG`
SAV0224-ermB-r		`AGTCAGGTCGACGCGACTCATAGAATTATTTCCTCCC`
PCR and DNA Sequencing confirmation Primers
SAermB375R		`TTTGGTTTAGGATGAAAGCATTC`
SA0222-fseq500	SAV0222	`CATGACACTTGTAGCATTTGTG`
SAV0222-fseq1000	SAV0222	`GTGCAACACCACCTGCAATG`
MUT0223CF		`GGAATTATGATCTGCCCCGAGAC`
SAMUT0223R400	SAV0225	`CTGATAACACACCACCCATAAAGACGTC`
SA0224ermB222F		`GCCATACCACAGATGTTC`
MUT0224AF		`AGTCAGGGATCCTTGAACCGTTCAATATCTTGC`
acuc-R	SAV1735	`CGGCTATGGACACACTGTAAATG`
SAV1652-F		`GTGCTGATGGAGTTATGGC`
SAV0225-R	SAV0225	`CAAGCCGCTCAGTACAACAACG`
SAV0222-F	SAV0222	`GGAATCGTTGCTTTCTATGAAG`
Primers for RT-qPCR
16S-RTF	SAVrRNA16	`GTGGAGGGTCATTGGAAACT`
16S-RTR:	SAVrRNA16	`CACTGGTGTTCCTCCATATCTC`
pflB-F	SAV0226	`ACGACTTCAACACGATGTCTAC`
pflB-R	SAV0226	`TTCTGCTGGACGGCTTAAAT`
ADH-F	SAV0605	`AATTGGAGACCGTGTGTCTATC`
ADH-R	SAV0605	`TTCAGCCATTGCACCATCTA`
rocA-F	SAV2554	`GCTGAAATGGGTGGTAAAGATG`
rocA-R	SAV2554	`CCAAACGCTGACGTTACAATAG`
DHA-1439F	SAV1439	`GCTATTGACCAAGGTGGAACTA`
DHA-1439R	SAV1439	`GGACTGCTCCTGGTTGATTT`
ccpA4F	SAV1736	`CAGGAACAAATGGTAAGGATGC`
ccpA4R	SAV1736	`TCTCCACCTACTAAAGCAAATGA`
uhpTA2-F	SAV0222	`CCCATCGGTGATTGCATTACT`
uhpTA2-R	SAV0222	`CCGGCTCTTCCCAAATTTCT`
SAV0225RTF	SAV0225	`CACCCTCATCCTAAGCGTAAA`
SAV0225RTR	SAV0225	`GCATCGAACTCTGCAACTAATC`
SAV0224RTF	SAV0224	`GATGCTTCAGCCACTCATAGAA`
SAV0224RTR	SAV0224	`TGCCAATGTCAGGCGTATAG`
SAV0223RTF	SAV0223	`ACCAGTAGACCATGCACAATTA`
SAV0223RTR	SAV0223	`TGACAAGATGCTAAGCTACGG`