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. 2018 Dec 12;13(12):e0208560. doi: 10.1371/journal.pone.0208560

Fig 6. Drought stress and methyl jasmonate treatment affect PtaJAZ3 and PtaRAP2.6 expression.

Fig 6

(a) Transcript levels of PtaJAZ3 and PtaRAP2.6 in response to PEG-induced drought stress. Poplar roots grown in control and PEG liquid media (see experimental procedures) were used for qRT-PCR analysis. Values are show as mean ± standard error of mean (SEM) (n = 2). Asterisks represent statistical significant levels of differences between control and PEG treatment (P< 0.05) calculated using Student’s t test. (b) PtaJAZ3 and PtaRAP2.6 are induced in response to methyl jasmonate (MeJA) treatment but the induction of both genes was abolished in presence of proteasome inhibitor, MG-132. Transcript levels were analyzed in poplar root samples treated with 0.2% DMSO (mock), 100 μM MeJA and 100 μM MeJA+ 80 μM of MG-132 (see experimental procedures). Values show as mean ± standard error of mean (SEM) (n = 2). Asterisks represent the treatments that are statistically different from the mock treatment (P< 0.05) calculated using Student’s t test.