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. 2018 Nov 30;14(11):e1007390. doi: 10.1371/journal.pgen.1007390

Fig 4. Tmk1 signaling is required for hyphal regeneration and correct transcriptional response.

Fig 4

A. Microscopic changes observed 1 h after injury in the WT, Δtmk1 and Δtmk3. Hyphae were stained with lactophenol cotton blue and examined by light microscopy. Arrows point to the new hyphae. B. The graph shows the percentage of hyphae that regenerate upon injury in each strain. Three independent experiments were performed for each treatment, counting 50 hyphae in each case. Bars represent the mean ± s.e.m. A one-way ANOVA test, followed by Tukey Honest Significant Differences was used. Different letters indicate significant differences (P < 0.05). C. Venn diagrams showing the overlap between up-regulated and down-regulated genes that respond to injury in the WT, Δtmk1 and Δtmk3 strains. D. Heat map showing the expression profile of the Injury-Responsive Tmk1 dependent (IRK1) genes and their behavior following injury in each strain (FDR < 0.05; Fold-change > 1). E. Heat map with enriched GO biological process terms, showing the percentage of genes belonging to each category (FDR <0.01**; FDR <0.05*). The number in parenthesis after each GO term indicates the total number of genes in each category.