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. 2018 Nov 30;14(11):e1007390. doi: 10.1371/journal.pgen.1007390

Fig 6. The expression of RGS correlates with the initial stages of regeneration.

Fig 6

A. Microscopic changes observed after injury. Hyphae were stained with lactophenol cotton blue and examined by light microscopy. Regenerating hyphae were photographed 15, 30 minutes, and 1, 2, and 5 hours after injury. Scale bar = 10 μm. Images are representative of the morphological stage of the hyphal population at the indicated time. B. Relative mRNA expression of a Het domain, a Nacht domain encoding genes, and the rad5, and cmk1 genes. The expression is relative to DNA polymerase encoding gene (Id. 53190), which expression does not vary under the tested conditions. Graphs show the results of four biological and three technical replicates. Error bars represent ± s.e.m. A one-way ANOVA test, followed by Tukey test were used to determine significant differences, indicated by different letters (P < 0.05).