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. Author manuscript; available in PMC: 2019 Jul 6.
Published in final edited form as: J Org Chem. 2018 May 24;83(13):7239–7249. doi: 10.1021/acs.joc.8b00855

Figure 9.

Figure 9.

Biosynthesis of ADR-GlyU. (A) HPLC analysis of one-pot reaction starting with 10a and UTP (*impurity in commercial UTP) after 12-h incubation with (i) no enzymes; (ii) Mur16; (iii) Mur16 and Mur17; (iv) Mur16, Mur17, and LipO; (v) Mur16, Mur17, LipO, and LipP; (vi) Mur16, Mur17, LipO, LipP, and Mur18; (vii) Mur16, Mur17, LipO, LipP, Mur18, and Mur19; and (viii) 17a standard. (B) HPLC analysis of one-pot reaction starting with 10b, UTP, and 12 after 12-h incubation with (i) no enzymes; (ii) Mur16; (iii) Mur16 and LipO; (iv) Mur16, LipO, and LipP; (v) Mur16, LipO, LipP, and Mur18; (vi) Mur16, LipO, LipP, Mur18, and Mur19. A260, absorbance at 260 nm.