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. 2018 Dec 12;13(12):e0208978. doi: 10.1371/journal.pone.0208978

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© 2018 Hussack et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) Cartoon diagram illustrating the two V_HH-Fc formats used with varying hinge length and composition. (B) Purified V_HH-Fcs were analyzed by SDS-PAGE under non-reducing (N) and reducing (R) conditions. (C) Representative V_HH-Fc SEC elution profile from a Superdex 200 column. Inset, plot of SEC elution volumes for all V_HH-cFcs and V_HH-hFcs. (D) Representative SPR sensorgrams showing 30 min (1800 s) dissociations of V_HH-Fc fusions flowing over immobilized TcdB_1751-2366 surfaces. (E) Dissociation rate comparison of V_HH-Fcs with human or camelid hinges.