Figure 6.

S100A11 regulates monocyte recruitment in vivo. (a) WT and S100a11 KO mice were infected intraperitoneally with T. gondii, and the presence of inflammatory monocytes and neutrophils at the site of infection was analyzed by flow cytometry on day 5 post infection. (b) Average frequency and absolute numbers of monocytes in T. gondii-infected WT and S100a11 KO mice on day 5 post infection. The data shown are representative of four independent experiments each involving 4–6 age- and sex-matched mice. (c) CCL2, IL-12p40, and IFN-γ production were measured in the peritoneal cavity on day 5 post infection by ELISA. The data shown are representative of three independent experiments (d) CCL2, IL-12p40, and IFN-γ production were measured in sera on day 5 post infection by ELISA. Each symbol represents an individual experimental muse (e) WT, S100a11 KO, and Caspase-1/11 DKO mice were infected intraperitoneally with T. gondii, and the release of S100A11 was analyzed by ELISA on day 5 post infection. The data shown are representative of three independent experiments. (f) Survival of WT (black circles) and S100a11 KO (open circles) mice infected with T. gondii (20 cysts per mouse). The data shown are representative of four independent experiments each involving 6–8 age- and sex-matched mice. (b-e) Unpaired two-tailed Student’s t test and (f) Mantel-Cox tests were utilized for the statistical analysis, ns = not significant