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. Author manuscript; available in PMC: 2020 Jan 1.

Published in final edited form as: Acta Biomater. 2018 Nov 7;83:221–232. doi: 10.1016/j.actbio.2018.11.010

Figure 4. — (a) Fibrillar collagen microstructure. Top row: Collagen fiber microstructure shown by second harmonic two-photon microscopy in nanocomposite hydrogel with 1% TSB POSS, 1% PEG POSS, or 7.5mM CaCl2 crosslinking. Bottom row shows corresponding quantification of collagen fibers within a 30 μm3 VOI segmented in Imaris software for each type of hydrogel. Plots show measurements of fiber diameter (x-axis) and volume (y-axis). The pseudocolor scale also indicates diameter of fibers. (b) Quantification of collagen diameter. *: > 1% PEG POSS gel, p < 0.05; #: > 1% TSB POSS gel, p < 0.05. (c) Quantification of collagen volume. *: > 1% PEG POSS gel, p < 0.05; #: > 1% TSB POSS gel, p < 0.05.

Figure 4. — (a) Fibrillar collagen microstructure. Top row: Collagen fiber microstructure shown by second harmonic two-photon microscopy in nanocomposite hydrogel with 1% TSB POSS, 1% PEG POSS, or 7.5mM CaCl2 crosslinking. Bottom row shows corresponding quantification of collagen fibers within a 30 μm3 VOI segmented in Imaris software for each type of hydrogel. Plots show measurements of fiber diameter (x-axis) and volume (y-axis). The pseudocolor scale also indicates diameter of fibers. (b) Quantification of collagen diameter. *: > 1% PEG POSS gel, p < 0.05; #: > 1% TSB POSS gel, p < 0.05. (c) Quantification of collagen volume. *: > 1% PEG POSS gel, p < 0.05; #: > 1% TSB POSS gel, p < 0.05.