Table 1.
Cuticular proteins (CuPs) identified in the virus–insect vector interaction studies presented in this review
| Virus species/genus | Vector — species | Transmission mode | CuPs identifiera — other name | Protein familyb (subfamily) | Approaches | Reference |
|---|---|---|---|---|---|---|
| ZYMV/Potyvirus | Aphid — M. persicae | Noncirculative | AAO63549 | CPR (RR-2) | Urea extraction of aphid CuPs, 1-D & 2-D gel electrophoresis, Far-western blot, MS analyses | [17] |
| AAL29466 | CPR (RR-2) | |||||
| AAZ20451 | CPR (RR-1) | |||||
| AAZ20447 | CPR (RR-2) | |||||
| CaMV/Caulimovirus | Aphid | Noncirculative | ND | ND | Biochemical characterization, Stylet immunolabeling | [40•] |
| CaMV/Caulimovirus | Aphid — A. pisum, M. persicae | Noncirculative | MG188739 — Stylin-01 | CPR (RR-1) | Stylet immunolabeling, Colocalization, in vitro competition assays, RNAi | [15••] |
| MG188741 — Stylin-01 | CPR (RR-1) | |||||
| CMV/Cucumovirus | Aphid — M. persicae | Noncirculative | DQ108938 — Mpcp4 | CPR (RR-1) | YTH | [22] |
| CMV/Cucumovirus | Aphid — A. pisum | Noncirculative | ND | CPR (RR-2) | Peptide array (RR-2 proteins) | [25] |
| TuYVc/Polerovirus | Aphid — M. persicae | Circulative | NDd | ND | Whole cell lysate (aphids), 1-D & 2-D gel electrophoresis, Far-western blot & MS analyses | [16] |
| CYDV-RPV/Polerovirus | Aphid — S. graminum | Circulative | gi:193647865 | CPR (RR-2) | Genetics coupled to 2-D-DIGE & MS analyses | [18••] |
| gi:193706873 | NDd | |||||
| gi:193647875 | CPR (RR-2) | |||||
| gi:193582403 | CPR (RR-2) | |||||
| BYDV-GPV/Luteoviridaee | Aphid — R. padi | Circulative | gi:288558725 — Cp 62 precursor | CPR (RR-2) | iTRAQ & MS analyses | [20] |
| NP_001156154.1 — Cp 5 precursor | CPR (RR-2) | |||||
| RSV/Tenuivirus | Planthopper — L. striatellus | Circulative-Propagative | KC485263 — CPR1 | CPR (RR-1) | YTH, Chemiluminescent co-IP, Colocalization, GST pull-down, RNAi | [13••] |
| RSV/Tenuivirus | Planthopper — L. striatellus | Circulative-Propagative | XM_014390248.1 — Cuticle protein A3A like | CPR (RR-2) | YTH | [26] |
| JAS02196.1 | Tweedle |
Given accession numbers from original studies.
Classified using CutProtFam-Pred (http://aias.biol.uoa.gr/CutProtFam-Pred/).
Formerly BWYV-FL1 (beet western yellows virus).
published accession number does not correspond to a CuP according to databases and CutProtFam-Pred.
Unassigned member in the family Luteoviridae. CuP: cuticular protein; ND: not determined; MS: mass spectrometry; RNAi: RNA interference; DIGE: difference gel electrophoresis; iTRAQ: isobaric tags for relative and absolute quantification; co-IP: co-immunoprecipitation; YTH: yeast two-hybrid. ZYMV: zucchini mosaic virus; CaMV: cauliflower mosaic virus; CMV: cucumber mosaic virus; TuYV: turnip yellows virus; CYDV: cereal yellow dwarf virus; BYDV: barley yellow dwarf virus, RSV: rice stripe virus.