Skip to main content
. 2018 Dec 6;9:2803. doi: 10.3389/fimmu.2018.02803

Figure 1.

Figure 1

HHV-6A infection induces expression of MSRV-Env in different cell types. (A) HSB2 cells, cord blood mononuclear cells (CBMC) and U87 cells were infected with HHV-6A at MOI 1 or incubated with the mock control for 24 h and MSRV env expression was analyzed by RT-qPCR. The values are expressed relatively to those in mock-infected cells and error bars represent SEM of 3 independent experiments. (B) Cytofluorometric analysis of U87 and HSB-2 cells infected or not with HHV-6A for 24 h at MOI 0.1 and stained by anti-MSRV-Env mAb, followed by anti-mouse Ig-Alexa 647 (green: non-infected cells + secondary Ab staining; orange: non-infected cells + complete staining; blue: infected cells + secondary Ab staining, pink: infected cells + complete staining). (C,C') U87, (D,D') SH-SY5Y, (E,E') HSB-2, and (F,F') peripheral blood mononuclear cells (PBMC) were either incubated with mock preparation (C–F) or infected at MOI 0.1 (C'–F') and analyzed 24 h later by immunofluorescence using anti-MSRV-Env mAb, followed by anti-mouse Ig-Alexa 555 (red staining). HHV-6A induced syncytia formation of adherent infected cells (C',D', *). DAPI (blue staining) was used to visualize cell nuclei (Bar = 50 μm). Data are representative of at least three independent experiments.