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. 2018 Dec 6;9:2803. doi: 10.3389/fimmu.2018.02803

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Copyright © 2018 Charvet, Reynaud, Gourru-Lesimple, Perron, Marche and Horvat.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MSRV-ENV and MSRV-GAG were expressed in HHV-6A infected cells. (A,C) U87 and (B,D) HSB-2 cells were infected with HHV-6A during 24 h. Cells were fixed and stained for MSRV-ENV (GN_mAb_Env01) (A,B) or MSRV-GAG (GN_mAb_Gag06) (C,D) followed by anti-mouse-Alexa 555 (red staining). HHV-6 staining was revealed using biotinylated anti-HHV-6-p41 mAb, followed by streptavidin-FITC (green staining). HHV-6A infected cells expressing either MSRV-ENV or MSRV-GAG were pointed by arrowhead. DAPI (blue staining) was used to visualize cell nuclei. Bar = 50 μm. Bottom left frames (B,D): higher magnification of cell pointed by arrowhead.