Skip to main content
. 2018 Dec 6;9:2803. doi: 10.3389/fimmu.2018.02803

Figure 5.

Figure 5

Engagement of CD46-SCR3 and/or CD46-SCR4 trigers intracellular and cell surface MSRV-Env expression. Several CD46 ligands known to bind different CD46 SCR domains were used to stimulate U87 cells: (A,G) anti-CD46 rabbit polyclonal antibody, (B) C3b component of complement (50 mM), (C) Anti-SCR1 Tra2.1 (75 μg/ml), (D) anti-SCR1 MC20.6 (75 μg/ml), (E) anti-SCR3-4 GB24 (75 μg/ml), (F) anti-SCR4 MEM-258 (50μg/ml). The expression of MSRV-Env was detected using anti-MSRV-Env mAb-coupled to Alexa 555 by immunofluorescence analysis (red staining, arrowhead), including DAPI (blue staining) to visualize cell nuclei (bar = 50 μm) (A–F), or by cytofluorometry (G), using anti-MSRV-Env mAb followed with anti-mouse-Alexa 647 (blue: cells stimulated with anti-CD46 + secondary Ab staining, pink: cells stimulated with anti-CD46 followed by complete anti-MSRV-Env staining, green: cells stimulated with isotype control mAb + staining with secondary Ab; orange: cells stimulated with isotype control mAb followed by complete anti-MSRV-Env staining). Good cell viability was observed in all stimulation conditions. (H) Schematic representation of CD46 SCR domains recognized with utilized CD46 ligands, having a different effect on the induction of MSRV-Env.