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. 2018 Dec 6;9:2803. doi: 10.3389/fimmu.2018.02803

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Copyright © 2018 Charvet, Reynaud, Gourru-Lesimple, Perron, Marche and Horvat.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Induction of MSRV-Env expression is dependent of CD46-Cyt1 isoforme. (A) CD46-Cyt1 and CD46-Cyt2 mRNA expression levels in si-CD46-Cyt1-treated U87 cells normalized to si-control treated cells, analyzed by RT-qPCR (relative expression). Error bars, mean ± SD of three experiments, statistical test used: nonparametric KS test (n.s. P > 0.1, ****P < 0.0001, vs. untreated condition) (B–E) MSRV-Env expression in U87 cells, treated with either si-RNA control (B,D) or and si-Cyt1 (C,E), and either left non-infected (B,C) or infected with HHV-6A (MOI 0.1) for 24 h (D,E). MSRV-Env expression was determined using anti-MSRV-Env mAb, followed by anti-mouse-Alexa 555 (red staining) cellular nuclei were visualized using DAPI (blue staining), bar = 50 μm.