Figure 8.

HHV-6A-induced MSRV-Env triggers human TLR4. (A) Schematic presentation of experimental conditions. HEK cells co-expressing human TLR4 (hTLR4) and TLR4 activation reporter gene (SEAP), were stimulated with either HHV-6A, supernatants from HHV-6A infected or non-infected U87 cells or co-cultured in presence of U87 cells previously infected with HHV-6A or not. hTLR4 activation was monitored colorimetrically using a SEAP reporter gene placed under the control of an NF-κB inducible promoter. (B) Data are expressed as corrected absorbance at 620 nm (absorbance obtained for HEK-blue + HHV-6A, mean: 0.27 ± 0.3, was subtracted from the absorbance obtained for other experimental conditions). Statistical analysis was performed using t-test on means ± SEM from three independent experiments (***P < 0.001, ****P < 0.0001, vs. uninfected conditions).