Figure 10.

Rhopr-IR knockdown suppresses an IR-associated phosphorylation signaling cascade. The fat bodies of unfed dsIR-injected 5th instar insects displayed decreased Akt and FOXO phosphorylation compared with insects injected with dsARG, when measured 4 days post-injection (n = 7 insects per trial). Protein phosphorylation in 5th instar fat bodies was visualized through Western blot analysis, probing for anti-phospho-Akt (pAkt), anti-pGSKβ3 and anti-pFOXO (primary antibodies, 1:1000), and visualized using Chemiluminescence.