Figure 7.

The Rhopr-IR pathway is activated by mammalian insulins. (A) After injecting unfed 5th instar insects with either 0.1 or 1 μg porcine insulin (Pins or PI), an increase in the phosphorylation of Akt was detected within fat bodies as observed through Western blot analysis (probing for phospho-Akt and visualized using Chemiluminescence), compared to injection with glucose-free saline. (B) Porcine insulin (0.1 μg) significantly stimulated the phosphorylation of Akt relative to saline (with or without glucose) following injection of unfed 5th instar insects and a 30 min incubation with all treatments (n = 5 insects per trial). The experiment was repeated 3 times (n = 5 insects per trial) and results are shown as means + std error. **p < 0.01 indicates statistically significant difference, inferred using one-way ANOVA with Dunnett's multiple comparisons test.