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. 2018 Dec 6;9:745. doi: 10.3389/fendo.2018.00745

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Copyright © 2018 Defferrari, Da Silva, Orchard and Lange.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lipid and carbohydrate measurements in recently fed Rhopr-IR knockdown insects. 5th instar R. prolixus were injected with 1 μg of dsARG or dsIR in 1 μL of ultrapure water and fed on defibrinated rabbit blood 3 days after injections. Hemolymph and fat body samples were collected 4 days post-feeding. Significance of results is inferred comparing dsIR-injected insects to dsARG-injected insects. (A) Increase in hemolymph lipid level in dsIR-injected insects (n = 26 dsARG, n = 27 dsIR). (B) Decrease in fat body lipid content in dsIR-injected insects (n = 25 dsARG, n = 20 dsIR). (C) Hemolymph carbohydrate levels (n = 22 dsARG, n = 22 dsIR). (D) Fat body carbohydrate content (n = 10 dsARG, n = 9 dsIR). Results are shown as means + std errors. An asterisk (*) indicates statistically significant differences between dsIR and dsARG injected insects.