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. 2018 Dec 6;9:2880. doi: 10.3389/fimmu.2018.02880

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Copyright © 2018 van der Zwan, van der Meer-Prins, van Miert, van den Heuvel, Anholts, Roelen, Claas and Heidt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alloreactivity of EBV B8/FLR T cell clone 4D5 against HLA-C^*01:02. (A) EBV B8/FLR T cell lines (n = 9; 1A11 shown) and T cell clones (n = 6; 4D5, clone 1, and clone 19 shown) were stimulated with a panel of HLA-C expressing SALs after which IFNγ production was measured. EBV B8/FLR T cell clone 4D5 showed alloreactivity against HLA-C^*01:02. (B) One EBV B8/FLR T cell line and four EBV B8/FLR T cell clones (4B8 and 4D5 shown) were stimulated with a panel of SALs and EBV-LCLs expressing HLA-B^*08:01, HLA-C^*01:02, and HLA-B^*44:02 alleles after which IFNγ production was measured. The range of the ELISA standard curve: 5–5120 pg/ml; Ho, homozygous; He, heterozygous. Bars represent duplicate values with standard deviation of the mean.