Figure 3.

Generated HCMV HLA-C^*06:02/TRA T cell lines and clones, and the HCMV HLA-C^*07:02/CRV T cell clone are cytolytic against their target cells. (A) FACS plots of HLA-C^*06:02/TRA tetramer staining at time of sorting and 2 weeks after expansion of the tetramer-positive CD8+ T cells. (B) Two HCMV C^*06:02/TRA T cell lines (1A3 shown) and two T cell clones, and HCMV C^*07:02/CRV T cell clone LH were stimulated with SALs and EBV-LCLs expressing HLA-C^*06:02 or C^*07:02 loaded with viral peptide. (C) Cytotoxicity of one HCMV C^*06:02/TRA T cell line (1A3 shown) and two T cell clones, and HCMV C^*07:02/CRV T cell clone LH was tested against⁵¹Cr-labeled SALs, 721.221 cells and EBV-LCLs expressing the virus-specific restriction HLA allele alone or loaded with viral peptide. The range of the ELISA standard curve: 5–5120 pg/ml. Ho, homozygous; He, heterozygous. Bars represent triplicate values with standard deviation of the mean.