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. 2018 Dec 6;9:2880. doi: 10.3389/fimmu.2018.02880

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Copyright © 2018 van der Zwan, van der Meer-Prins, van Miert, van den Heuvel, Anholts, Roelen, Claas and Heidt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alloreactivity of HCMV C^*06:02/TRA T cell lines against HLA-C^*03:02. (A) Two HCMV C^*06:02/TRA T cell lines (1A3 shown) and two T cell clones (1F12 shown), and HCMV C^*07:02/CRV T cell clone LH were stimulated with a panel of EBV-LCLs after which IFNγ production was measured. (B) Three HCMV C^*06:02/TRA T cell lines (1A3 shown) and five T cell clones (10C1 shown) were stimulated with EBV-LCLs and PHA blasts, obtained from two different donors, expressing the recognized allo-HLA-C^*03:02 allele and the virus-specific restriction allele HLA-C^*06:02 loaded with viral peptide as a positive control. SALs expressing HLA-C^*06:02 loaded with viral peptide were also included as a positive control. The range of the ELISA standard curve: 5–5120 pg/ml. Bars represent duplicate values with standard deviation of the mean.