Figure 5.

HCMV C^*06:02/TRA T cell lines and clones are cytotoxic against HLA-C^*03:02. (A) Cytotoxicity of two HCMV C^*06:02/TRA CD8+ T cell lines (1A3 shown) and one CD8+ T cell clone (10C1) was tested against⁵¹Cr-labeled EBV-LCLs and PHA blasts, obtained from three different donors, expressing the recognized allo-HLA-C^*03:02 allele and the virus-specific restriction HLA allele loaded with viral peptide as a positive control. SALs expressing HLA-C^*06:02 loaded with viral peptide were also included as a positive control. (B) The CD8+ T cell lines and clone were incubated with an anti-CD8 blocking antibody prior to co-culture with target cells and specific lysis was measured. Bars represent triplicate values with standard deviation of the mean.