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. 2018 Dec 6;9:3001. doi: 10.3389/fmicb.2018.03001

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Copyright © 2018 Urrutia, Sabag, Valenzuela, Labra, Álvarez and Santiviago.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adherence and internalization of S. Typhimurium strains in D. discoideum. Individual infection assays were conducted to evaluate the adherence and internalization of different S. Typhimurium strains in D. discoideum AX4. (A) Relative internalization after 1 h of infection at 22°C. (B) Relative adherence after 30 min of co-incubation at 4°C. All values (internalization and adherence) were calculated as CFU_t ₌₀/CFU_inoculum and further normalized to the value of the wild-type strain. Graphs show mean values ± SD from at least 7 independent assays. Statistical significance of differences in internalization or adherence between each strain and the wild type was determined using a one-way ANOVA with Dunnett’s test (^∗∗P < 0.01; ^∗∗∗P < 0.001).