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Primers used in this study.
| Primer name | Sequence |
|---|---|
| tatABC_(H1+P1) | AGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTGTTGGTGCAGGCTGGAGCTGCTTC |
| tatABC_(H2+P2) | GCGGTTGTGTTTAGTCTTCAGTGTGCTCGGCCTTTTCGGTCATATGAATATCCTCCTTAG |
| tatABC_Out5 | GAGCGGGTCATTCTTACTCG |
| tatABC_Out3 | TTCGTTCCGGTCAGTAGCAT |
| pBAD_Forward | ATGCCATAGCATTTTTATCC |
| pBAD_Reverse | GATTTAATCTGTATCAGG |
Underlined sequences anneal to the 5′ or 3′ end of the antibiotic-resistance cassette in template vector pCLF4 (KanR, GenBank accession number EU629214).
