Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 6;12:914. doi: 10.3389/fnins.2018.00914

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Pozner, Schray, Regensburger, Lie, Schlötzer-Schrehardt, Winkler, Turan and Winner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Neuronal differentiation and characterization (A) Schematic representation of the differentiation of iPSCs into neuronal cultures. (B) Representative images of CTRL (left; CTRL-1a) and SPG11 (right; SPG11-1b) 4 weeks differentiated neurons stained for GFAP and Tuj-1. (C) Quantification of percentage of GFAP⁺/DAPI⁺ cells in CTRLs and SPG11. (D) qPCR data of 4 weeks differentiated neurons. RNA from four different iPSCs clones was used as negative control and it is indicated as a dashed line at onefold change. (E) Representative images Ctip2 (red) and Tuj-1 (green) of CTRL and SPG11. Quantifications represent pooled data of CTRLs (CTRL-1, CTRL-2) and SPG11 (SPG11-1, SPG11-2, SPG11-3) neurons. iPSCs, induced pluripotent stem cells; NPCs, neural progenitor cells. Scale bar = 50 μm in (B) and 20 μm in (E). ^∗P < 0.05.