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. 2018 Dec 12;3(6):e00633-18. doi: 10.1128/mSphereDirect.00633-18

TABLE 3.

Bacteria and plasmids used in this study

Strain or plasmid Description Reference(s)
or source
Bacterial strains
    S. aureus SH1000 Derivative of strain 8325-4, containing functional rsbU 37, 38
    S. aureus SH1000 (NsaSA208E) NIS-resistant derivative of SH1000 10
    S. aureus SH1000 (NsaSA105T) NIS-resistant derivative of SH1000 10
    S. aureus MW2 Community-acquired MRSA strain 3
    S. aureus MW2 (NsaSR209I) NIS-resistant derivative of MW2 10
    S. aureus MW2 (NsaSG210D) NIS-resistant derivative of MW2 10
    E. coli SA08B Cloning host that modifies cloned DNA for introduction into wild-type S. aureus strains 34
    E. coli BTH101 Host strain for two-hybrid assays 36
Plasmids
    pRMC2 E. coli/S. aureus shuttle vector containing the Pxyl/tet promoter for
tetracycline-inducible gene expression in S. aureus 		35
    pIMAY E. coli/S. aureus shuttle vector, for allelic replacement in S. aureus 33
    pMUTIN4 Suicide vector for insertional inactivation of genes in S. aureus 32
    pUT18 Vector for two-hybrid analyses. Enables C-terminal fusion of T18 domain of adenylate cyclase
to protein of interest		 36
    pUT18C Vector for two-hybrid analyses. Enables N-terminal fusion of T18 domain of adenylate cyclase
to protein of interest		 36
    pKT25 Vector for two-hybrid analyses. Enables N-terminal fusion of T25 domain of adenylate cyclase
to protein of interest		 36
    pKNT25 Vector for two-hybrid analyses. Enables C-terminal fusion of T25 domain of adenylate cyclase
to protein of interest		 36