Scheme 1. Substrate scope for Mb(H64V,V68A,H93S)[(Co(ppIX)]-catalyzed cyclopropanation of vinylarenes with EDA.a.
a Reaction conditions: 10 mM olefin, 20 mM EDA, E. coli whole cells expressing Mb variants (OD600 = 80) in 50 mM phosphate buffer (pH 7.2). b Based on GC conversion. c For trans-(1S,2S) product. d Using 5 mM olefin and 10 mM EDA. Enantiomeric excess not determined.