Table 3.
Catalytic activity and selectivity of selected Mb(H64V,V68A) variants in the reaction with 4-(dimethylsilyl)styrene (3a) and EDA.a
 | |||||
|---|---|---|---|---|---|
| Entry | Protein | Cofactor | % yieldb |
% 3bc |
% 3cc |
| 1 | Mb(H64V,V68A) | Fe(ppIX) | 41 | 6 | 92 |
| 2 | Mb(H64V,V68A,H93S) | Fe(ppIX) | 36 | 4 | 96 |
| 3 | Mb(H64V,V68A) | Mn(ppIX) | 18 | 5 | 94 |
| 4 | Mb(H64V,V68A,H93A) | Mn(ppIX) | 36 | 3 | 96 |
| 5 | Mb(H64V,V68A,H93F) | Mn(ppIX) | 35 | 4 | 96 |
| 6 | Mb(H64V,V68A) | Co(ppIX) | 37 | 33 | 49 |
| 7 | Mb(H64V,V68A,H93S) | Co(ppIX) | 44 | 74 | 24 |
| 8 | Mb(H64V,V68A,H93F) | Co(ppIX) | 25 | 63 | 10 |
| 9 | Mb(H64V,V68A) | Rh(mpIX) | 33 | 6 | 94 |
a
Reaction conditions: 10 mM 3a, 10 mM EDA, E. coli whole cells expressing Mb variant (OD600 = 40) in 50 mM phosphate buffer (pH 7.2).
b
Based on GC conversion using calibration curves with isolated 3b and 3c.
c
The remainder to 100% corresponds to double insertion product(s).