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. 2018 Nov 1;8(11):2238–2253.

Figure 6.

Figure 6

Downregulation of NOB1 suppresses GBM cell proliferation and colony formation, promotes cell apoptosis, and impedes cell migration and invasion. T98 and U251 cells were treated with NOB1 siRNA or NC siRNA. After incubation for different times, transfected cells were used in the following assays. A. Western blot analysis was conducted to determine NOB1 protein expression in T98 and U251 cells transfected with NOB1 siRNA or NC siRNA. *P<0.05 vs. NC siRNA. B, C. Proliferation and colony formation capacities in the abovementioned cells were examined using CCK-8 and colony formation assays, respectively. *P<0.05 vs. NC siRNA. D. The percentage of apoptotic cells was evaluated in NOB1 siRNA or NC siRNA transfected- T98 and U251 cells via flow cytometry analysis. *P<0.05 vs. NC siRNA. E, F. Transwell migration and invasion assays were used to determine the effects of NOB1 knockdown on the migration and invasion in T98 and U251 cells. *P<0.05 vs. NC siRNA.