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. 2018 Nov 1;8(11):2238–2253.

Figure 7.

Figure 7

NOB1 reintroduction reverses the effects of miR-744 on GBM cells in vitro. T98 cells were co-transfected with miR-744 mimics and pc-NOB1 or pcDNA3.1, while miR-744 inhibitor along with NC siRNA or NOB1 siRNA was transfected into U251 cells. A. Western blot analysis was carried out to measure NOB1 protein expression. *P<0.05 vs. miR-NC. **P<0.05 vs. miR-744 mimics+pcDNA3.1. #P<0.05 vs. NC inhibitor. **P<0.05 vs. miR-744 inhibitor+NC siRNA. B-F. Proliferation, colony formation, apoptosis, migration and invasion in T98 and U251 cells treated as above were examined using CCK-8 assay, colony formation assay, flow cytometry analysis, and transwell migration and invasion assays, respectively. *P<0.05 vs. miR-NC. **P<0.05 vs. miR-744 mimics+pcDNA3.1. #P<0.05 vs. NC inhibitor. **P<0.05 vs. miR-744 inhibitor+NC siRNA.