Table 2.

Nucleotide diversity values (π) for passaged populations for each gene and for the concatenated sequences.

	Concatenated sequence	TGB1	TGB2	TGB3	CP
Sp13	0.0012 ± 0.0003	0.0015 ± 0.0007	0.0006 ± 0.0003	0.0008 ± 0.0004	0.0012 ± 0.0003
PS5	0.0012 ± 0.0003	0.0011 ± 0.0003	0.0005 ± 0.0002	0.0010 ± 0.0004	0.0017 ± 0.0008
Sp13∗	0.0013 ± 0.0002	0.0011 ± 0.0003	0.0010 ± 0.0004	0.0019 ± 0.0006	0.0015 ± 0.0003
PS5∗	0.0013 ± 0.0003	0.0007 ± 0.0002	0.0024 ± 0.0012	0.0026 ± 0.0017	0.0009 ± 0.0002
H30	0.0022 ± 0.0005	0.0017 ± 0.0005	0.0024 ± 0.0011	0.0027 ± 0.0016	0.0026 ± 0.0008
KLP2	0.0016 ± 0.0003	0.0014 ± 0.0004	0.0027 ± 0.0014	0.0012 ± 0.0006	0.0014 ± 0.0004

Sp13^∗ and PS5^∗ correspond to PepMV-Sp13 and PepMV-PS5 from mixed infections. Nucleotide diversity (π) was estimated with MEGA7 using the K2P model and was expressed as the average number of nucleotide substitutions per site between each pair of sequences. Standard error estimates are shown and were calculated using 1000 bootstrap replicates.