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. 2018 Nov 15;10(11):3701–3712.

Figure 2.

Figure 2

The low expression of RNF152 in CRC cell lines and the effect of RNF152 knockdown in CRC cells. A. QPCR analysis of RNF152 mRNA levels in 6 CRC cell lines and 2 patients’ normal mucosa. ACTB mRNA was used as an internal control. B. QPCR analysis of RNF152 expression level in HCT116 cells transduced with 2 shRNA of RNF152 TET-ON virus in the absence and presence of doxycycline. Student’s t-test; ***P<0.001. C. The proliferation of shRNF152 TET-ON HCT116 cells in the absence and presence of doxycycline was analyzed by a CCK-8 assay. Knocking down of RNF152 in HCT116 showed no difference of CRC cell growth. The cell numbers were analyzed every day for 5 days. RNF152: Ring Finger Proteins 152; CRC: colorectal cancer; QPCR: quantitative-polymerase chain reaction; sh: short hairpin RNA.