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. 2018 Nov 15;10(11):3701–3712.

Figure 3.

Figure 3

The effect of RNF152 ectopic expression in CRC cells in vitro. A. QPCR analysis of RNF152 expression level in HCT116, SW620 and LOVO cells transduced with RNF152-expressing PLVX lentivirus (containing the FLAG tag). The overexpression efficiency of RNF152 was significant. Student’s t-test; **P<0.01. B. The proliferation of RNF152-ectopic expression HCT116, SW620 and LOVO cells was analyzed by CCK-8 assays. Overexpression of RNF152 in HCT116, SW620 and LOVO cells significantly decreased CRC cell growth in vitro. The cell numbers were analyzed every day for 5 days. Student’s t-test; ***P<0.001. C and D. The proportion of apoptotic cells as determined by flow cytometry analysis in RNF152-ectopic expression HCT116 and SW620 cells. RNF152 could promote cell apoptosis. Student’s t-test; ***P<0.001. RNF152: Ring Finger Proteins 152; CRC: colorectal cancer; QPCR: quantitative-polymerase chain reaction.