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. 2018 Nov 15;10(11):3465–3480.

Figure 4.

Figure 4

Evaluation of late (21 days) chondrogenic differentiation. A. Alcian blue and immunostaining were used to detect sGAG and type II collagen deposition respectively in pellets from each group after 21 days of chondrogenic induction and ImageJ software was used to quantify the immunohistological intensity. Magnification: (a) 100× and (b) 200×. The experiments were conducted in triplicate. *P < 0.05 compare with other groups. B. Immunofluorescence staining was used to detect type X collagen deposition after 21 days of chondrogenic induction and DAPI was used as a nuclear marker. ImageJ software was used to quantify the immunofluorescence intensity. Magnification: 100×. The experiments were conducted in triplicate. *P < 0.05 compare with other groups. C. Real-time PCR was used to evaluate the chondrogenic marker genes (Sox 9, COL2A1 and ACAN) and hypertrophic marker genes (RUNX2, ALP and MMP13) expression. The experiments were conducted in triplicate. *P < 0.05 compare with other groups.