Figure 5.

Evaluation of osteogenic and adipogenic differentiation. A. After a 21-day incubation in osteogenic medium, osteogenesis was evaluated using alkaline phosphatase (ALP) staining. Magnification: 100×. The experiments were conducted in triplicate. *P < 0.05. B. After a 21-day incubation in adipogenic medium, adipogenesis was evaluated using Oil Red O (ORO) staining. Magnification: 100×. C. Real-time PCR was used to evaluate the expression of ALP. The experiments were conducted in triplicate. D. Real-time PCR was used to evaluate the expression of adipogenic marker genes (LPL and PPARγ). The experiments were conducted in triplicate. ***P < 0.05 of any two groups.