Figure 6.

Involvement of MAPKs and Wnts in expansion, early (7 days) and late (21 days) chondrogenic differentiation of SDSCs and WJ-MSCs expanded on PL and dECM. Western blot was used to detect the expressions of MAPK signals (JNK, ERK and p38 at both phosphorylation and total protein levels) and Wnts signals (Wnt3a and Wnt5a). p-JNK and p-38 were actively involved in the expansion and late chondrogenesis, respectively. β-actin was used as the internal control and ImageJ software was used to quantify the bands. The experiments were conducted in triplicate. *P < 0.05 compare with other groups. **P < 0.05.