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. 2018 Nov 15;10(11):3395–3412.

Figure 2.

Figure 2

Silencing ZEB1-AS1 expression suppresses glioma cancer cell proliferation in vitro and tumor growth in vivo. A. The inhibitory efficiency of siZEB1-AS1 transfection on the expression of ZEB1-AS1 was measured by qRT-PCR assay. B. Silencing ZEB1-AS1 by siZEB1-AS1 significantly inhibited proliferation of U87 cells at 2 d, 3 d, and 4 d. Cell proliferation was detected by CCK-8 assay. C. Cell proliferation was detected by colony formation assay in U87 cells transfected with siZEB1-AS1 or siNC. D. Cell migration and invasion was determined by transwell assays. Silencing ZEB1-AS1 by siZEB1-AS1 significantly inhibited the migratory (left, upper panel) and invasive (left, lower panel) ability of U87 cells after transfected with siZEB1-AS1. E. U87 cells were transfected with siZEB1-AS1 or siNC and tumors were collected from SCID mice subcutaneously injected into the right hind flank with the treated cells. Representative images of tumors 4 weeks after subcutaneous xenografting were showed. F. The tumor volume was analyzed once every four days. G. The quantification of tumor weights (n = 6). H. The quantification of pulmonary nodules in each group within 8 weeks after tail vein injection. siNC: siRNA negative control; siZEB1-AS1: ZEB1-AS1 stably deleted cells. *P < 0.05, **P < 0.01, siZEB1-AS1 versus siNC group. All values are expressed as mean ± SD of three replicates.