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. 2018 Nov 15;10(11):3395–3412.

Figure 5.

Figure 5

ZEB1-AS1 promotes glioma cancer cells proliferation, migration and invasion through inhibition of miRNA-200c/141 and activation of ZEB1. U87 cells were transfected with siNC, siZEB1-AS1 and vector, siZEB1-AS1 and ZEB1, miR-200s and vector, and miR-200s and ZEB1, respectively. qRT-PCR assay was performed to detect the expression of ZEB1-AS1 (A) and ZEB1 mRNA (B) in treated U87 cells. (C and D) Cell proliferation was detected by CCK-8 assay and colony formation assay in treated U87 cells. (E) Representative images of transwell assays in treated U87 cells. Total magnification of all images is 200 ×. (F and G) Quantification of migrated and invaded cells were presented. *P < 0.05, **P < 0.01, siZEB1-AS1 + ZEB1 versus siZEB1-AS1 + Vector; #P < 0.05, ##P < 0.01, miR-200s + ZEB1 versus miR-200s + Vector. siNC: siRNA negative control; siZEB1-AS1 + Vector: ZEB1-AS1 stably deleted cells transfected with vector; siZEB1-AS1 + ZEB1: ZEB1-AS1 stably deleted cells transfected with ZEB1; miR-200s + Vector: U87 cells transfected with the mixtures of miR-200c and miR-141; miR-200s + ZEB1: U87 cells transfected with miR-200s and ZEB1. Data are shown as mean ± SD based on at least three independent experiments.