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RRM2B gene silencing promotes mitochondrial permeability transition pore (MPTP) opening. Live-cell fluorescence microscopy analysis of MPTP activity in MCF7 cells expressing vector, shNS or shRRM2B. All mitochondria are stained red with MitoTracker Red CMXRos (left panel). Healthy cells are stained green with calcein AM (middle panel), which is concentrated in the mitochondria. Merged images are presented on the right panel.
