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. 2018 Nov 15;10(11):3413–3429.

Figure 5.

Figure 5

JAK2 overexpression inhibits OGD/R-induced autophagy and apoptosis in C2C12 myoblasts. C2C12 myoblast cells were transfected with lentivirus carrying JAK2 plasmid (JAK2-OE) or a negative control plasmid (NEG), exposed to normoxia (control group) or OGD/R. A. Light microscope photographs of C2C12 myoblast cells (JAK2-OE and/or NEG) exposed to normoxia (control group) or OGD/R, the scale was shown in corresponding picture. B. Representative immunoblot images and relative quantification of JAK2; C. Representative immunoblot images and relative quantification of P62 and LC3, collectively an index of autophagy flux; D. Representative immunoblot images of Caspase 3, Bcl-2, Bax, cleaved Caspase 3 and relative quantification of Bax/Bcl-2 ratio and cleaved Caspase 3/Caspase 3 ratio, collectively an index of apoptosis level. Quantification expressed as fold induction over control group (assumed as 1), data were determined by densitometric analysis from at least 3 independent experiments after normalization by β-actin (Means ± SD; *P < 0.05, **P < 0.01 versus control group; #P < 0.05, ##P < 0.01 versus NEG+OGD/R group).