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. 2018 Nov 15;10(11):3413–3429.

Figure 7.

Figure 7

Representative protein expression of the JAK2/STAT3, AKT/mTOR, and ERK1/2 pathways. Western blot analysis of the total protein fraction from C2C12 myoblasts transfected with lentivirus carrying JAK2 plasmid (JAK2-OE) or a negative control plasmid (NEG), with normoxia (control group) or OGD/R treatment. The inhibitors LY294002 (10 μM) and U0126 (10 μM) were added to the medium 30 min before each experiment. (A) Representative immunoblot images of phosphorylated JAK2, total and phosphorylated STAT3; (B) Representative immunoblot images of total and phosphorylated mTOR, total and phosphorylated AKT; (C) Representative immunoblot images of total and phosphorylated ERK1/2; (D) Representative immunoblot images of LC3 and cleaved Caspase 3. Relative quantification of p-JAK2 and the ratios of p-STAT3/STAT3 (E), the ratio of p-mTOR/mTOR (F), the ratio of p-AKT/AKT (G), the ratio of p-ERK(1/2)/ERK(1/2) (H), and LC3 II and cleaved Caspase 3 (I), determined by densitometric analysis. Values were expressed as the means ± SD for three independent experiments. *P < 0.05, **P < 0.01 versus control group; #P < 0.05, ##P < 0.01 versus NEG+OGD/R group; aP < 0.05, aaP < 0.01 versus JAK2-OE+OGD/R group.